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cell culture inserts  (Nest Biotechnology)

 
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    Nest Biotechnology cell culture inserts
    Cell Culture Inserts, supplied by Nest Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cell culture inserts/product/Nest Biotechnology
    Average 86 stars, based on 1 article reviews
    cell culture inserts - by Bioz Stars, 2026-06
    86/100 stars

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    Image Search Results


    Characterization and fabrication of nasal mucosal epithelial cell sheets derived from EGFP rats. (A) Schematic representation of the fabrication process for nasal mucosal epithelial cell sheets using EGFP rats. (B) Phase-contrast images 12 days after primary explant culture. (C) Phase-contrast micrographs of subcultured cells 7 days after seeding onto temperature-responsive culture dishes. (D) Macroscopic appearance of a cell sheet harvested by reducing the incubation temperature to 20 °C. (E) Colony-forming assay of cells harvested from the nasal mucosal epithelial cell sheet. (F) Fluorescence microscopy. Representative images of EGFP (green) and DAPI (blue) in the cell sheet. Scale bar = 50 μm. (G) Immunohistochemical staining for pan-cytokeratin. A representative cross-sectional image of the nasal mucosal epithelial cell sheet showing positive immunoreactivity for pan-cytokeratin (AE1/AE3). Scale bar = 25 μm.

    Journal: Regenerative Therapy

    Article Title: Characterization of the role and fate of nasal mucosal epithelial cell sheets in middle ear regeneration

    doi: 10.1016/j.reth.2026.101130

    Figure Lengend Snippet: Characterization and fabrication of nasal mucosal epithelial cell sheets derived from EGFP rats. (A) Schematic representation of the fabrication process for nasal mucosal epithelial cell sheets using EGFP rats. (B) Phase-contrast images 12 days after primary explant culture. (C) Phase-contrast micrographs of subcultured cells 7 days after seeding onto temperature-responsive culture dishes. (D) Macroscopic appearance of a cell sheet harvested by reducing the incubation temperature to 20 °C. (E) Colony-forming assay of cells harvested from the nasal mucosal epithelial cell sheet. (F) Fluorescence microscopy. Representative images of EGFP (green) and DAPI (blue) in the cell sheet. Scale bar = 50 μm. (G) Immunohistochemical staining for pan-cytokeratin. A representative cross-sectional image of the nasal mucosal epithelial cell sheet showing positive immunoreactivity for pan-cytokeratin (AE1/AE3). Scale bar = 25 μm.

    Article Snippet: The cells were then seeded onto temperature-responsive cell culture inserts (CellSeed) at a density of 4 × 10 4 cells/cm 2 and cultured in KCM for 7 days.

    Techniques: Derivative Assay, Incubation, Fluorescence, Microscopy, Immunohistochemical staining, Staining